CD21^−/lo B cells contain antinuclear and anticytoplasmic clones. The frequencies of antinuclear (A) and anticytoplasmic (C) clones in CD21⁺ and CD21^−/lo B cells from HD, RA, and CVID patients are shown. P values calculated using a paired t test are indicated when significant. (B) ANAs expressed by CD21^−/lo B cells showed a diversity of nucleolar (RA01 21⁻ κ181, RA01 21⁻ κ137, RA14 21⁻ κ15, and RA14 21⁻ κ83), nucleolar and speckled (RA19 21⁻ κ127), and speckled (CVID 21⁻ κ12) antinuclear staining patterns. (D) Anticytoplasmic CD21^−/lo B cells can react with well-defined intracellular structures, which include mitochondria (RA01 21⁻ κ140, and CVID321 21⁻ κ82), the Golgi (RA14 21⁻ κ18), and cytoplasmic fibers (RA19 21⁻ κ61, RA14 21⁻ κ62, and RA01 21⁻ κ157 that is likely to recognize actin).