TLR9 signaling by CpG ODN induces autocrine cytokine production by B-CLL cells. (A) Kinetics of cytokine production by purified B-CLL cells in cultures with or without CpG 2006 at the indicated time points (hours). The data shown are representative results from 1 of 3 experiments with CLL cells from different patients. (B) Aggregated results (n = 5, mean ± SD) of cytokine production by purified B-CLL cells or unpurified PBMCs containing more than 70% of B-CLL cells from patients 1 to 5 in cultures with or without CpG 2006 for 72 hours. *P < .01, each cytokine production by B-CLL cells in cultures with versus without CpG 2006.