CpG-B ODN-induced apoptosis in B-CLL cells is treatment time- and dose-dependent. (A) B-CLL cells were cultured with various doses of the CpG ODNs for 5 days. Data are the mean ± SD from 3 independent experiments with B-CLL cells from different CLL patients. (B) B-CLL cells were exposed to the indicated CpG ODNs (3 μg/mL) for different time lengths, washed, and cultured in media for a total of 7 days. Data are the mean ± SD from 3 independent experiments with B-CLL cells from different CLL patients. (C) B-CLL cells were exposed to different doses of CpG-B ODNs for different time lengths, washed, and cultured in media for a total of 7 days. Data are representative results from 1 of 2 experiments. The dashed lines indicate the required treatment time and/or dose of CpG ODN to reach the 50% decrease of B-CLL cells.