Localization and quantitative analysis of hemostatic proteins in the lung. (A) Micrograph panel showing (columns) immunostaining for PAI-1, tissue factor (TF), TFPI and thrombomodulin (TM) in (rows) healthy controls, septic baboons (E coli), and septic baboons treated with compstatin (CS) during the first (E coli + CS T0) or the second (E coli + CS T + 5) stage. The colors of the each antigen and nuclear counterstaining are shown on the top row. ct indicates convolulted tubules; and g, glomerulus. Magnification bars, 50 μm. (B) Scatter-plot representations of MFI of images collected for the above-mentioned proteins and experimental conditions. PAI-1: whole-field MFI of at least 15 images; all other immunostainings: 20 ROI (alveolar capillaries) in 15 images (200 spots). (C) Histogram representation of mRNA expression for PAI-1, TF, TFPI, and TM. Values indicate the mean ± SEM of fold over β-actin housekeeping gene. In panels B and C, data are presented as mean ± SEM. One-way ANOVA with Dunnett multicomparison test. *P < .05; **P < .001; ***P < .001 compared with E coli group.