Immunofluorescence confocal imaging and quantitative analysis of fluorescence intensity in kidneys stained for several complement pathway proteins. (A) Micrograph panel showing on columns immunostaining for mannose-binding lectin (MBL) C3b, TCC (C5b9), CD55, and CD59 in (rows) healthy controls, septic baboons (E coli), and septic baboons treated with compstatin (CS) during the first (E coli + CS T0) or the second (E coli +CS T + 5) stage. The color of each antigen is shown on the upper row. To facilitate recognition of microscopical structures, green autofluorescence (first column) or nuclear staining channels (columns 2-5) were collected. Magnification bar, 50μm. (B) Scatter-plot representation of mean fluorescence intensity (MFI) of the images collected for the above-mentioned proteins and experimental conditions. MBL: 20 peritubular capillary ROI in 15 images (200 spots); all other images: whole-field MFI of at least 15 images for each experiment. Scatter-plot data are shown as mean ± SEM. One-way ANOVA with Dunnett multicomparison test; ***P < .001 compared with the E coli group.