Comparison of signaling pathways in IMMU-114–sensitive and –resistant cells. (A) Western blot analyses of ERK1/2 and JNK1/2 phosphorylation on cell extracts of IMMU-114–sensitive and –resistant cell lines after mAb treatment. (B) Effect of ERK, JNK, and ROS inhibitors on IMMU-114–mediated apoptosis in Raji. NAC (5mM) was used to block the ROS. U0126 (10μM) blocks MEK phosophorylation and the ERK1/2 pathway. SP600125 (10μM) blocks the JNK pathway. (C) Effect of siERK and siJNK RNAs on the expression of ERK and JNK proteins. Error bars represent SD of 3 replicates. (D) Western blot analyses of ERK and JNK pathways in CLL patient samples.