Sequential analyses of NRAS, NPM1, and WT1 mutations in patient 33 showing acquisition of novel mutations in these 3 genes at relapse. (A) Sequential follow-up of mutation pattern at Patient 33. (B) Wild-type NRAS at diagnosis (top) and GGT>TGT at NRAS codon 12 at relapse (bottom). Arrows indicate the location of the mutation. (C) Wild-type NPM1 at diagnosis (top); NPM1 mutation with a TCTG insertion at relapse (bottom). The boxed tetranucleotides indicate the location of the mutation. (D) Wild-type WT1 at diagnosis (top) and nt1335/1336 (+11 bp) on exon 7 at relapse (bottom).