Csf2rb, Il1rl1, and Gata2 are expressed in both mouse and human leukemic cells. (A) Representative FACS plots of Csf2rb and Il1rl1 staining in lineage-negative, c-Kit⁺ ScaI⁺ (LKS⁺) and c-Kit⁺, ScaI⁻ (LKS⁻) bone marrow and spleen from nonleukemic Cbfb^+/+ mice, and leukemic cells from the spleen of mice expressing a conditional allele of Cbfb-MYH11 (Cbfb^+/56M, Mx1-Cre). N ≥ 3 for Cbfb^+/56M, Mx1-Cre. Expression of Csf2rb (B) and Il1rl1 (C) in the peripheral blood of leukemic Cbfb^+/56, Mx1 Cre⁺ and control littermate mice. The control group contained Cbfb^+/+, Mx1-Cre⁻; Cbfb^+/56M, Mx1-Cre⁻; and Cbfb^+/+, Mx1-Cre⁺ mice. All mice were treated with pI:pC in the same way. (D) Western blot analysis of Gata2 expression in the peripheral blood of a nonleukemic Cbfb^+/+ adult mouse, 2 different leukemic adult mice expressing Cbfb-MYH11, and the human inv(16) AML-derived ME-1 cells. The bottom panel shows α-tubulin expression using the same blot. (E) Reverse transcription–PCR analysis using primers specific for human CSF2RB in ME-1 cells. An arrow indicates the band of the expected size. (F) FACS plot of ME-1 cells stained for C-KIT and IL1RL1. Percentage of cells in each gate is given.