AML in myr-AKT mice. (A) Hematoxylin and eosin–stained histopathology sections of spleen, liver, and BM from a representative myr-AKT mouse with AML. Details on image acquisition can be found in supplemental Methods. (B) Flow cytometric analysis of BM and spleen cells from a representative myr-AKT mouse with AML. All were gated for GFP⁺ cells. (C) AML and T-cell lymphoma are transplantable, whereas MPD is not. Kaplan-Meier survival curves representing secondary transplantations of splenocytes from myr-AKT mice with MPD, thymocytes from myr-AKT mice with T-cell lymphoma, or splenocytes from myr-AKT mice with AML injected into the tail veins of sublethally irradiated C57 Bl/6 mice. MPD secondary transplantation mice were followed for 120 days with no evidence of disease. (D) Western blot of representative splenocyte and thymocyte lysates from diseased myr-AKT mice and a WT age-matched control mouse. (E) Intracellular flow cytometry on GFP-gated thymocytes and GFP-gated CD71^hic-Kit^hi splenocytes from a myr-AKT mouse and a WT age-matched control mouse.