Verification of human and mouse microarray data. To verify differential expression between monocyte subsets revealed by microarray analysis, 9 genes were amplified from (A) human and (B) mouse monocyte subsets by qPCR. Results from qPCR (■) and microarray (□) are depicted as relative fold change, where negative values are more highly expressed on the CD16⁻ human or Ly-6C⁺ mouse monocyte subset and positive values are more highly expressed on the CD16⁺ human or Ly-6C^lo mouse subset. qPCR results were in good agreement with microarray data.