A2t attenuates macrophage TNF-α production in response to LPS. Macrophages were treated with 10 ng/mL LPS for 24 hours, with or without a 6-hour pretreatment with 2nM A2t. Controls were treated with 2nM A2t alone for 30 hours. Supernatants were collected at 24 hours after LPS treatment, and TNF-α was measured by luminex.