Figure 4
Figure 4. HSPCs in the SVF originated in the BM. (A) Experimental scheme. After lethal irradiation, the mice underwent transplantation with 5 × 106 GFP+ BM (BMT) cells. At 16 weeks after transplantation, the SVF was sampled and analyzed. (B) CFC assay of SVF from GFP+ BMT mice. Representative colonies were viewed by bright field, phase-contrast, and fluorescence, and then merged. Scale bars, 100 μm. (C) The colonies were classified and counted by morphologic criteria.

HSPCs in the SVF originated in the BM. (A) Experimental scheme. After lethal irradiation, the mice underwent transplantation with 5 × 106 GFP+ BM (BMT) cells. At 16 weeks after transplantation, the SVF was sampled and analyzed. (B) CFC assay of SVF from GFP+ BMT mice. Representative colonies were viewed by bright field, phase-contrast, and fluorescence, and then merged. Scale bars, 100 μm. (C) The colonies were classified and counted by morphologic criteria.

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