Phenotype of the different MSCs. Figure displays phase-contrast photomicrographs that depict the morphologic appearance of MSC lines and primary MSCs derived from the marrow of a patient with CLL. Cells were imaged in medium using a phase-contrast microscope (Model ELWD 0.3; Nikon) with a 10 × /0.25 NA objective lens. Images were captured with a Nikon D40 digital camera (Nikon Corp) with the use of Camera Control Pro software (Nikon); when necessary, Adobe Photoshop 9.0 (Adobe Systems) was used for image processing.