Blockade of TSP1/CD36 rescues inhibition of endothelial cell chemotaxis and angiogenesis by 8CPT. (A) HMVEC chemotaxis assay. Analysis of cells migrating to VEGF (200 pg/mL) after treatment with 8CPT (2.5μM), anti-TSP1 antibody (A4.1), 8CPT and anti-TSP1 (2 μg/mL), isotype control antibody (IgM, 2 μg/mL), or 8CPT and isotype control. Bars represent normalized means ± SD (n = 4 experiments, each with 4 replicates). ns, not significant; *P < .05; ***P < .001. (B) In vivo Matrigel plug angiogenesis assay. Mice were injected with Matrigel supplemented with VEGF (200 pg/mL); VEGF, 8CPT (2.5μM), and isotype control antibody; or VEGF, 8CPT, and anti-CD36 (FA-152) antibody. Microvessel density was counted, as described in “Methods.” Bars show mean ± SE (n = 5). *P < .05.