Figure 5
Activation of DCs does not abrogate FIX tolerance induced by intramuscular injection of AAV1 in BALB/c mice. Eight- to 10-week-old BALB/c mice received intramuscular injection of 3 × 1011 vg of AAV1-hFIX alone (■) or with LPS and anti-CD40 antibody (□; n = 5 for each cohort). Plasma was collected every 4 weeks after AAV and LPS plus anti-CD40 antibody injection, and measured for hFIX antigen (A) and anti-hFIX IgG antibodies (B) by ELISA. Data shown are mean ± SEM.
