Effects of MCFD2 deletion mutations on LMAN1 binding. (A) Diagram of MCFD2 deletion constructs used in the study. SS indicates signal sequence; L, linker; and M, myc tag. The N-terminal domain is defined as the first 42 amino acids after the signal sequence. (B) Immunofluorescence staining of the wild-type (WT), Δ40, and the Δ50 N-terminal deletion mutants, as well as the D129E missense mutant MCFD2. (C) co-IP of various N-terminal deletion mutants with LMAN1. (D) co-IP of ΔL1, ΔL2, and ΔEF2-N mutants with LMAN1. Cell lysates were immunopreciptated with anti-myc (myc), anti-LMAN1 (L), and anti-MCFD2 (M).