C57BL/6 syngeneic islet transplantation in BM. (A) Nonfasting glycemia of STZ-treated severely diabetic C57BL/6 mice transplanted with 500 equivalent islets into the BM (n = 24). (B) Left, Nonfasting glycemia of STZ-treated severely diabetic C57BL/6 mice transplanted in BM with 500 equivalent islets. BM of right femur was removed 9 months after transplantation (arrow; n = 6). Values shown are means ± SEM. Right, Dithizone staining of right femur BM contents after removal at 9 months after transplantation. (C) Histologic appearance (×10) of islets in BM 3 months after transplantation. Immunofluorescent images show insulin staining (red). Scale bar 200 μm. (D) Nonfasting glycemia reduction in the 8 weeks after transplantation with 500 equivalent islets according to transplant site. Intraliver: islets infused through the portal vein, n = 10. Intra-BM: islets infused directly into the BM, n = 10. Data are expressed as box plots of mean glycemia of the single weeks. Statistical analysis was performed by tests of repeated measures ANOVA.