Anti-A20 Id mAb can inhibit A20 tumor cell growth in vivo. (A) Balb/c mice were inoculated with 107 A20 tumor cells subcutaneously and then treated intraperitoneally with saline, anti-A20 Id mAb, or an isotype control mAb (anti-38C13 Id) 3 hours later. Numbers in parentheses indicate animals cured by therapy. (B) Balb/c mice were inoculated with 107 A20 tumor cells. Mice were then treated with anti-A20 Id mAb either 3 hours after tumor inoculation (tumor size, 0 cm2) or when tumors reached 0.5, 1.0, or 1.5 cm2 (as indicated by the arrow). Numbers in parentheses indicate animals cured by therapy. Each line represents a group consisting of 10 mice. (C) FcRKO mice were inoculated with 106 A20 tumor cells. Mice were then treated with anti-A20 Id mAb 3 hours after tumor inoculation. Each line represents a group consisting of 10 mice. (D) CD8−/− mice were inoculated with 106 A20 tumor cells. Mice were then treated with anti-A20 Id mAb 3 hours after tumor inoculation. Each line represents a group consisting of 10 mice.
