Figure 1
![Figure 1. Anti-A20 Id mAb binds A20 tumor cells and inhibits A20 tumor growth in vitro. (A) A20 tumor cells were washed and stained with an AlexaFluor 647-labeled anti-A20 Id mAb or with an AlexaFluor 647-labeled isotype control mAb. (B) A20 tumor cells were stimulated with anti-A20 Id mAb (S) or an isotype control mAb (I) for 1 hour at 37°C. The proteins were lysed, separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, Western blotted, and probed for total tyrosine phosphorylation expression. (C) A20 cells or 38C13 cells were incubated in the presence of anti-A20 Id mAb. Cells were then pulsed with [3H]thymidine and harvested. Data are represented as mean ± SD of triplicate values.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/114/20/10.1182_blood-2009-05-223263/4/zh89990944580001.jpeg?Expires=1724201075&Signature=Ez-iCSHl3AJo~WNbRkkZPHqxMJ2T3djBFVoAotLa~Re71p5SlEeG3pJnJCgNH6C3TOi~-yHGH8OyYjhtRsV3jRyxGnBNnQelRuw04gUPsLUVEpUx0yGdcjWaxylXtdUaa~lc1UdwChcoalt0lV26IV3KAkBIN6s01uSoJiDSwxVxdA8CexvUiW2ACujjBP0Z4XlrKE7Y0Hnca4sOSK2UKixQCLAXsvi13zCg3tQAh-dFZOa3hjkasr6uj~ugodKGKIC9sZ2Qn91VMF0I7FDE4OafGOHDggX8AJpwPnXJTOC5XG-O16pRBPn44Y4CQvMtAC6zcKQhRf1DqEDaZxTJQw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Anti-A20 Id mAb binds A20 tumor cells and inhibits A20 tumor growth in vitro. (A) A20 tumor cells were washed and stained with an AlexaFluor 647-labeled anti-A20 Id mAb or with an AlexaFluor 647-labeled isotype control mAb. (B) A20 tumor cells were stimulated with anti-A20 Id mAb (S) or an isotype control mAb (I) for 1 hour at 37°C. The proteins were lysed, separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, Western blotted, and probed for total tyrosine phosphorylation expression. (C) A20 cells or 38C13 cells were incubated in the presence of anti-A20 Id mAb. Cells were then pulsed with [3H]thymidine and harvested. Data are represented as mean ± SD of triplicate values.
