Figure 4
Figure 4. CD56+ cells from human blood are enriched in γδ T cells. (A) Freshly isolated CD56+ cells were stained for CD3 (SK7-PerCP-Cy5.5), αβ TCR (WT31-FITC), and γδ TCR (11F2-PE) and analyzed with a flow cytometer. Using plots of CD3 against FSC, CD3+ cells were gated (left) and then selectively analyzed for αβ TCR or γδ TCR expression (right). Selective gating of CD3bright or CD3low cells (left) identified γδ T cells and αβ T cells, respectively (right). (B) Side-by-side analysis of CD56+ DCs and CD56+ γδ T cells showed that only γδ T cells but not DCs expressed γδ TCR.

CD56+ cells from human blood are enriched in γδ T cells. (A) Freshly isolated CD56+ cells were stained for CD3 (SK7-PerCP-Cy5.5), αβ TCR (WT31-FITC), and γδ TCR (11F2-PE) and analyzed with a flow cytometer. Using plots of CD3 against FSC, CD3+ cells were gated (left) and then selectively analyzed for αβ TCR or γδ TCR expression (right). Selective gating of CD3bright or CD3low cells (left) identified γδ T cells and αβ T cells, respectively (right). (B) Side-by-side analysis of CD56+ DCs and CD56+ γδ T cells showed that only γδ T cells but not DCs expressed γδ TCR.

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