Coculture of endothelial cells with tumor cells and blood elements induces expression of inflammation-associated genes including CCL5. (A) Expression map (red indicates strong expression; green, no expression) of genes that were significantly differentially expressed (P < .05, ANOVA, Benjamini-Hochberg posthoc test) in HMVECs cocultured as indicated. (B) Expression map of the highest up-regulated endothelial transcripts in HMVECs cocultured with blood elements and LS180 cells (+plat, leukos, LS180) compared with HMVECs cocultured only with leukocytes and platelets (+plat, leukos). Mean expression of 3 independent experiments is presented by the indicated color code (P < .05 by 2-tailed Student t test). (C) Fold changes of genes that were strongly induced in HMVECs cocultured with blood elements and LS180 cells compared with human umbilical vein endothelial cells (HUVECs) cocultured in the same condition. Genes that were differentially expressed between quiescent HMVECs and quiescent HUVECs were previously subtracted from the list (P < .05 by 2-tailed Student t test).