Selectin ligands on tumor cells mediate the inflammatory response of HMVECs. (A) E-selectin expression by quiescent HMVECs (left) and by HMVECs cocultured with LS180, PMNs, and monocytes (right). (B) E-selectin expression on HMVECs after the coculture with blood elements and LS180 or Colo205 cells in the presence of a P-selectin function-blocking antibody (10 μg/mL). (C) E-selectin expression on HMVECs cocultured with blood elements and LS180 or Colo205 cells, treated with O-sialoglycoproteinase (OSGPase) or sham treated. Mean ± SEM. **P < .01 by 2-tailed Student t test. (D) Representative flow cytometric data of E-selectin expression on HMVECs cocultured with HT-29 or Caco-2 cells either in the absence or presence of blood elements (PMNs, monocytes, and platelets). (E) Relative nuclear translocation of p65 in HMVECs cocultured with HT-29 cells, leukocytes, and platelets. Stimulation with TNF-α was used as a positive control of endothelial activation. Mean ± SEM of 3 independent experiments.