Anti-CMV reactivity of polyclonal Vδ2⁻ γδ T-cell lines isolated from CMV seronegative or seropositive blood donors. Whole Vδ2⁻ γδ T-cell lines generated by polyclonal activation were incubated on monolayers of CMV-infected or uninfected primary fibroblasts in the presence of an anti-CD107a mAb. After 6 hours, T cells were stained with an anti-Cδ mAb and analyzed by flow cytometry; representative raw data are presented in Figure S2. Shown here are the percentages of CD107a⁺ cells in γδ T cells for each studied donor.