Lymphocyte homing to splenic white pulp. (A) White pulp/red pulp architecture in spleens of Slc35c1−/− mice. Frozen sections of spleens from littermate control (+/+) and Slc35c1-deficient (−/−) mice were stained with anti–mSIGN-R1 mAb DSR1–14.2, which specifically detects macrophages in the marginal zone surrounding the white pulp. (B) Distribution of homed lymphocytes in white and red pulp. Wild-type splenic lymphocytes were fluorescently labeled and injected into the tail veins of littermate control (+/+) and Slc35c1-deficient (−/−) mice. At 3 hours later, spleens were collected, sectioned, and stained with mAb DSR1-14.2. (C) Lymphocyte homing to the white pulp in Slc35c1−/− mice. Homing of lymphocytes to the white pulp is expressed as the number of fluorescent cells per white pulp area. Data are means (± SD) from 3 experiments with a total of 6 mice per group and a minimum of 1 × 103 counted cells per spleen.
