Lymphocyte homing to the spleen. (A) Expression of fucosylated glycans in the spleen. Frozen sections of spleens from littermate control (+/+) and Slc35c1-deficient (−/−) mice were stained with biotinylated Aleuria autantia lectin (AAL) and secondary fluorescent streptavidin reagent to detect fucosylated structures. Insets show staining with secondary reagent only. (B) Lymphocyte homing. Lymphocytes obtained from LNs or spleens of wild-type mice were fluorescently labeled and injected into the tail veins of littermate control (+/+) and Slc35c1-deficient (−/−) mice. Spleens were collected 3 hours later. Following erythrocyte lysis, splenocytes were quantified and the percentage of fluorescent cells was determined by flow cytometry. Data are from 3 experiments, with a total of 9 spleens for each type of mouse.