American Society of Hematology

Figure 4

$Figure 4. Lymphocyte homing to LNs. Wild-type lymphocytes were fluorescently labeled and injected into the tail veins of Slc35c1−/− mice (−/−) or littermate controls (+/+). Three hours later, PLNs and MLNs were recovered by microdissection. The fractions of fluorescent cells in LN cell suspensions were determined by flow cytometry. Total LN cell numbers were analyzed with a cell counter. (A) Fraction of labeled cells in LN cell suspensions. (B) Total number of cells per LN. (C) Absolute number of homed lymphocytes per LN. Data are from 3 experiments, with a total of at least 42 PLNs and 18 MLNs for each group.$

Lymphocyte homing to LNs. Wild-type lymphocytes were fluorescently labeled and injected into the tail veins of Slc35c1^−/− mice (−/−) or littermate controls (+/+). Three hours later, PLNs and MLNs were recovered by microdissection. The fractions of fluorescent cells in LN cell suspensions were determined by flow cytometry. Total LN cell numbers were analyzed with a cell counter. (A) Fraction of labeled cells in LN cell suspensions. (B) Total number of cells per LN. (C) Absolute number of homed lymphocytes per LN. Data are from 3 experiments, with a total of at least 42 PLNs and 18 MLNs for each group.

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