Induction of primary T- and B-cell responses via αDEC-205-EBNA1 vaccination in immunodeficient mice reconstituted with human immune system components. Reconstituted mice were vaccinated intraperitoneally with PBS, Ig-EBNA1, or αDEC-205-EBNA1 using poly(I:C) as an adjuvant and boosted a month later. Two months after boost, bulk splenocytes were harvested, stimulated with either EBNA1 peptides or an influenza matrix protein 1 peptide library, and subjected to an IFN-γ ELISPOT assay. (A) Ratio of EBNA1-specific IFN-γ spots to nonspecific spots against the influenza peptides summarized for each vaccine group. Horizontal lines represent medians.(B) IFN-γ spots/105 splenocytes and EBNA1 IgM levels shown for 2 animals from each group. An index value of 1.1 or higher confirms the presence of anti-EBNA1 antibodies. Error bars represent SD.
