Immediate DC precursors are responsive to a decrease in number of differentiated DCs and DC precursors. Mixed BM chimera mice depicted in Figure 2A were treated with DT for the indicated period of time. (A) Splenic cDCs (CD11c^hiMHC-11⁺ cDCs) were analyzed for DNA content with propidium iodide at various time points after DT application, when the DC compartment was reduced (day 1 of DT treatment) and then expanding (days 5 and 11 of DT treatment). One representative mouse is shown for each time point. (B) Scatter plot summarizing the percentage of dividing (PI⁺) cDCs at different times points of DT administration from experiment shown in panel A (n = 7 for day 0, n = 4 for day 1, n = 5 for day 5, n = 4 for day 11 of DT treatment). Shown are individual mice, mean ± SEM. No statistically significant differences were observed (P = .406, ANOVA). (C top) Summarized gating strategy to identify pre-cDCs (full gating details on supplemental Figure 6); (bottom) scatter plot summarizing the number of DTR⁻ pre-cDCs in BM, blood, and spleen after DC depletion in mixed BM chimeras as depicted in Figure 2A. (D) Scatter plot showing the frequency of DTR⁻ pre-cDCs in the spleen after DT treatment. Note that DTR⁻ pre-cDCs are only approximately 20% of the total pre-cDC compartment before DT treatment. (E top) Summarized gating strategy to identify pro-DCs (full gating details on supplemental Figure 9); (bottom) scatter plot summarizing the number of pro-DCs in BM, blood, and spleen in CD11c.DOG mice after DC depletion. (F) Scatter plot showing the frequency of pro-cDCs in the spleen of CD11c.DOG mice after DT treatment. (C-F) Representative of 3 experiments showing 3 individual mice (*P < .05, ANOVA).