DC depletion in vivo by DT administration in CD11c.DOG mice. (A) Flow cytometry of splenocytes 24 hours after a single diphtheria toxin (DT) injection. Numbers indicate percentage of CD11c^hi MHC-II⁺ conventional DCs (cDCs). (B) Kinetics of cDC depletion and recovery in the spleen after a single DT injection (n = 4 for each time point). (C) Depletion kinetics and recovery of the CD8⁻CD4⁺, CD8⁺CD4⁻, and CD8⁻CD4⁻ subpopulations of cDCs in the spleen after a single DT injection (n = 4 for each time point). (D) Number of pre-DCs per spleen after DT treatment. Results are expressed as mean values ± SEM (n = 4). The gating strategy to identify pre-DCs is shown in supplemental Figure 1. (E) Continuous depletion of cDCs in the spleen after daily DT application. Results are expressed as mean value ± SEM (n = 4 for each time point). Shown is 1 representative of 3 for all experiments.