TACE inhibition impairs GM-CSF– and IL-4–mediated DC differentiation and induces macrophage/OC differentiation. (A) Monocytes were cultured in 24-well culture plates in the absence (top panels) or presence (middle panels) of GM-CSF and IL-4 in combination. TAPI-0 was added at 10μM together with GM-CSF and IL-4 in combination (bottom panels). After culturing for 5 days, cells were analyzed by flow cytometry. (B-C) Monocytes were cultured for 14 days in 24-well culture plates alone or in the presence of GM-CSF and IL-4 in combination without or with M-CSF and sRANKL. TAPI-0 was added at 10μM (bottom panels). Cells were fixed and visualized with Wright-Giemsa staining (B) or further incubated with FITC microspheres, followed by actin and nuclear staining (C). Green and red staining represent a microsphere and an actin, respectively.