Blocking antibodies to CD62L, CD44, and PSGL-1 but not LFA-1 significantly reduce the numbers of adoptively transferred monocytes entering inflamed retina. Freshly isolated EGFP bone marrow–derived monocytes (8 × 106) were injected intravenously into mice immunized 21 to 24 days previously with peptide to induce EAU. Mice were injected intravenously with 30 μg of control IgG or blocking antibody per mouse for 3 days. Numbers of circulating cells remaining in the blood were analyzed by flow cytometry (A). Transferred cells that had infiltrated the retinas were then counted by confocal microscopy of retinal whole mounts (B); **P < .01 compared with IgG control; Student t test; n was 12. Density of expression of adhesion molecules analyzed in both EAU (C) and control mice (D) after mAb treatment expressed as the geometric mean fluorescent index (Geo-MFI). *P < .05; **P < .01 compared with IgG control; Student t test; n was 3. Error bars represent SEM.
