Adoptively transferred monocytes in anti-CD62L–treated mice home to the spleen, and in anti-CD44–treated mice monocytes home to the lymph nodes in EAU but not control mice. Effect of monoclonal antibody treatment on trafficking of adoptively transferred monocytes into secondary lymphoid and other tissues in control and EAU mice by confocal microscopy. Freshly isolated EGFP bone marrow–derived monocytes (8 × 10⁶) were injected intravenously into mice immunized 21 to 24 days previously with peptide to induce EAU (A) or control mice (B). Mice were then injected intravenously with 30 μg of control IgG or blocking antibody per mouse per day for 3 days. Tissue samples were then snap frozen and cryosectioned, and the numbers of EGFP⁺ cells present in the tissues were enumerated (A,B). c-Node indicates cervical lymph node; m-Node, mesenteric lymph node. *P < .05; **P < .01; Student paired t test; n was at least 12 randomly chosen times 20 fields of view in tissue sections from 3 mice per group. Error bars represent SEM.