Depletion of circulating EGFP monocytes in the retinal vasculature by blocking mAb CD62L and CD44 is inflammation dependent. Freshly isolated EGFP bone marrow–derived monocytes (8 × 106) were injected intravenously into control mice or mice immunized 21 to 24 days previously with peptide to induce EAU. After 48 hours, cell trafficking in the retinal vasculature was analyzed by SLO. Retinal images were recorded for 10 minutes. Mice were then injected intravenously with 30 μg/mouse of rat anti–mouse antibody and recording continued for a further 20 minutes. Cells per minute passing through the same section of retinal vessel were compared before and after antibody treatment in control mice (A). (B) Recirculation of adoptively transferred cells was analyzed at 2-minute intervals (expressed as the number of transferred EGFP monocytes detected in the same section of retinal vessel) of control and EAU mice before and after antibody infusion. *P < .05; **P < .01; Student paired t test; n was at least 16 vessels from 3 mice in each group. Error bars represent SEM.
