Lack of response to TLR stimulations and reduced Ab production by B cells from HSP90b1 KO mice in response to Ag and a TLR ligand. (A-C) Splenic B cells were purified using magnetic beads coated with CD19 Ab from WT and KO mice. Cells were stimulated at 105 cells per well in a 96-well round-bottom plate with CpG (A), LPS (B), and PMA/ionomycin (C) at the indicated concentrations for 38 hours followed by pulsing with 3H-thymidine for 10 hours. Proliferation (cpm) was indexed by 3H-thymidine incorporation. Four experiments were performed with the similar findings. *P < .05; **P < .01. (D-F) Mice were immunized with OVA plus LPS in IFA, followed by measurement of serum anti-OVA IgG2c (D), IgG1 (E), and IgM (F) by ELISA (n = 4-6 mice per group). *P < .05; **P < .01. Error bars represent SEM.
