HIV-1 replication in IM-MDDCs is reduced by anti-DCIR. (Left panels) IM-MDDCs (2 × 105 cells) were initially treated with either a control Ab or a polyclonal anti-DCIR (10 μg per 1.5 × 105 cells). Next, cells were extensively washed to eliminate excess Ab and pulsed with NL4-3balenv (20 ng p24) for 2 hours at 37°C. After 3 washes with PBS to eliminate unbound virus, IM-MDDCs were maintained in complete culture medium supplemented with GM-CSF and IL-4. Cell-free culture supernatants were collected at the indicated time points and analyzed for the p24 content. Results obtained at 3 days after infection are displayed in the small inserts. Data shown represent the means plus or minus SD of triplicate samples from 2 different donors and are representative of 3 separate donors. Asterisks denote statistically significant data (*P < .05; **P < .01; ***P < .001). (Right panels) IM-MDDCs (1 × 105 cells) were first treated with either a control Ab or a polyclonal anti-DCIR (10 μg per 1.5 × 105 cells). Cells were next washed to eliminate excess Ab and pulsed with NL4-3balenv (10 ng p24) for 2 hours at 37°C. After 3 washes with PBS to eliminate unbound virus, IM-MDDCs were cocultured with autologous CD4+ T cells at a 1:3 ratio. Cell-free culture supernatants were collected at day 2 following initiation of the coculture and analyzed for the p24 content. Data shown represent the means plus or minus SD of triplicate samples from 2 distinct donors. Asterisks denote statistically significant data (**P < .01).
