Expression of DCIR in IM-MDDCs. Purified monocytes were cultured with GM-CSF and IL-4 for 7 days to derive IM-MDDCs. DCIR expression was determined by flow cytometric analysis after staining with a commercial PE-conjugated anti-DCIR monoclonal Ab. Expression of DCIR is shown as a dotted line, whereas the continuous line represents staining obtained with an isotype-matched irrelevant control Ab. Results shown are representative of 7 independent experiments.