Changes in methylation between diagnosis and relapse in patients with AML. (A) Categorical changes. Red boxes denote changes from unmethylated at diagnosis to methylated at relapse. Green boxes show genes methylated at diagnosis and unmethylated at relapse. White boxes denote no change in methylation status between diagnosis and relapse. NR, no result available. Genes were considered methylated if their methylation density exceeded the 95% confidence interval established in normal controls. (B) Differences in methylation densities. Red boxes denote an increase in methylation density at relapse of more than 10% when compared with diagnosis; green boxes show decrease of methylation density at relapse over 10%, and white boxes denote methylation changes within the 10% interval. Blasts, bone marrow blast count changes between the diagnosis and relapse. Red boxes denote an increase in blasts at relapse of more than 10% when compared with diagnosis; green boxes show a decrease of blasts at relapse over 10%; and white boxes denote changes of blasts within the 10% interval. Changes in blast counts did not correlate with methylation changes (Spearman nonparametric test not significant).