Effect of different kinase inhibitors on global tyrosine phosphorylation and the phosphorylation status of the positively regulating tyrosine residue in SFKs in CLL cells. Fresh PBMCs from 2 CLL patients or the cell line JVM-3 were cultivated for 2 hours and treated with either solvent control or the indicated concentrations of kinase inhibitors. Western blots were developed with primary antibodies directed against phosphotyrosine, pTyr416 of Src, Lyn, or tubulin. The inhibitor effects on patient cells are representative for 3 patient samples. indicate the position of SFK bands.