Figure 2
![Figure 2. Absence of recall proliferation in CD4+ T cells from FVIII plasmid plus anti-CD3–treated mice. CD4+ T cells were isolated by MACS from spleens of naive, anti-CD3 only, FVIII plasmid only, and FVIII plasmid + anti-CD3–treated mice 3 weeks after plasmid injection. A total of 1.0 × 105 CD4+ T cells were cocultured with irradiated 1.0 × 105 CD4− cells in 96-well round-bottom plates with or without the presence of FVIII at 10 U/mL for 72 hours, followed by adding 1 μCi of [3H]thymidine per well for the final 18 hours. *P < .05, compared with the group of FVIII plasmid only. Data shown are mean ± SD of counts per minute of [3H]thymidine incorporation in triplicate wells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/114/20/10.1182_blood-2009-05-217315/4/zh89990944690002.jpeg?Expires=1724833028&Signature=LeCL64I430IqAhoYrP4RN22fL6xF3vEaH7Wb5T3H9C2B58t9VO9~lFJ22v0CE~crfW-HXmV-3J9hu-zag5iHDaxvJkXEBCLXoMQqo4etXPBOu82mKRIQv4OOi~cv~XGuiOVDb8sWWr8QgxgNlxPAoH7Q-SxXWiUWOxnIxZI-bun5jsj1kDop5qSGGwoYbS0dUYKxS9xs8YpPwqAge4~34VWfbYmDch5kll9Fcn3oxFl9WY5M44F2LDkLCpPZwHOQztnd67TPTJ30L3GeKk27KbKrcMYiy7HqBm20HzgyPo8cgEC9l55~GhjFcAdoXoErWqvwLfxqtik2vZgf8hKx1A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Absence of recall proliferation in CD4+ T cells from FVIII plasmid plus anti-CD3–treated mice. CD4+ T cells were isolated by MACS from spleens of naive, anti-CD3 only, FVIII plasmid only, and FVIII plasmid + anti-CD3–treated mice 3 weeks after plasmid injection. A total of 1.0 × 105 CD4+ T cells were cocultured with irradiated 1.0 × 105 CD4− cells in 96-well round-bottom plates with or without the presence of FVIII at 10 U/mL for 72 hours, followed by adding 1 μCi of [3H]thymidine per well for the final 18 hours. *P < .05, compared with the group of FVIII plasmid only. Data shown are mean ± SD of counts per minute of [3H]thymidine incorporation in triplicate wells.
