Morphologic characteristics in musculus caput gastrocnemius 7 days after hindlimb ischemia operation. Images were taken from HE-stained 5-μm paraffin sections of mouse tissues and analyzed with Olympus Provis AX70 Microscope attached to Olympus ColorView 12 Camera. The software for imaging and analyses was AnalySIS (Soft Imaging System). (A) Increased regeneration shown in a hVEGF-D TG mouse on day 7 after hindlimb ischemia operation. Whole area showed late regeneration features as eosinophilic cytoplasm and internalization of nuclei. Bar, 200 μm. (B) Control group showed large area of necrosis with pale flocculated cytoplasm (*). Early stage of regeneration is represented by basophilic small myocytes with internalized nuclei (#). Bar, 200 μm.