Microarray and qPCR measurements of genes between TEL-AML1 and E2A-PBX1 subtypes. (A) Linear regression of expressions measured by qPCR (x-axis) versus those by microarray (y-axis) of NOTCH1, HDAC9, ANKRD15, CLIC5, FAT, NID2, PCLO, PTPRK, SOCS2, and TRIB2 in 5 TEL-AML1 samples and 5 E2A-PBX1 samples. Linear regression R2 of each comparison is shown above the corresponding curve. (B) The average expression level of 8 differentially expressed genes in 5 TEL-AML1 or 5 E2A-PBX1 samples measured by qPCR (top panel) or by microarray (middle panel) on the same Chinese ALL samples or on white children's samples of the same subtypes (bottom panel). The genes were ordered by the difference between the average expression values of TEL-AML1 and E2A-PBX1 samples determined by qPCR (from low to high). The height of each bar represents the average expression level of a gene in a sample group, and the whisker represents the standard deviation. The 1-sided t test P values between the gene expression values of the 2 ALL subtypes are indicated above the paired bars for each gene.
