Preexposure to live mf diminishes the NF-κB binding capacity of p50 and p65 following poly I:C and LPS activation. (A) Unexposed mhDCs activated with either LPS or poly I:C for 3 hours. (B) mhDCs were exposed to live mf for 48 hours and then activated with either media alone, poly I:C, or LPS for an additional 3 hours. Nuclear extracts were prepared, and NF-κB activation was measured using an ELISA with OD as the readout. Each line represents an independent experiment.