The main features of mDC-pDC cooperation are found also in PBMC. (A) Percentage of CD40⁺ mDCs and pDCs in unstimulated PBMCs (□) was compared after treatment with LPS (■) or CpG (▩). (B) TNF-α–positive mDCs and pDCs in PBMCs were determined using intracellular cytokine staining of unstimulated PBMCs (□) or PBMCs treated with LPS (■) or CpG (▩). (C) Percentage of intracellular TNF-α⁺ mDCs and pDCs in unstimulated PBMCs (□) or PBMCs exposed to E coli fixed bacterial particles (■). All data are from a representative experiment of 3 performed.