microRNA expression pattern in WM and healthy donors. (A) miRNA analysis has been performed on total RNA isolated from BM CD19⁺ WM cells, normal bone marrow (NBM)– and peripheral blood (PBMC)–derived CD19⁺ counterparts, and WM cell line (BCWM.1). Heat map was generated after unsupervised hierarchic cluster analysis of all unfiltered data. Differential expression of miRNA is shown by the intensity of red (up-regulation) versus blue (down-regulation). (B) Supervised hierarchic clustering analysis was performed using ANOVA test. Differential expression of miRNA patterns is shown by the intensity of red (up-regulation) versus blue (down-regulation). (C) Differential distribution of each indicated miRNA in all WM patients (WM) compared with healthy donors (HDs): mean values were compared using Mann-Whitney U rank sum test; bars indicate standard errors; P value (*) for each miRNA is indicated.