American Society of Hematology

Figure 3

$Figure 3. The DR− Tregs that produce IL-17 express CCR6 ex vivo. (A) Percentages of CCR6+ cells in gated ex vivo naive Tresps (n = 4), memory Tresps (n = 4), DR− Tregs (n = 6) and DR+ Tregs (n = 6). (B) CCR6− and CCR6+ fractions of DR− Tregs or memory Tresps were FACS-sorted and stimulated for 5 days with αCD3/αCD28 and IL-2 in the presence of IL-1β/IL-6. The percentages of IL-17+ cells at the end of the culture is shown (n = 4). (C) Cells were stimulated in the presence of IL-1β/IL-6 or TGFβ, and analyzed for IL-17 secretion by ELISA. At the end of the culture, CCR6+DR− Tregs were stained for intracellular IL-17 versus FoxP3 (D) or IFNγ (E). Data are representative of 4 independent experiments. **P < .01; *P < .05.$

The DR⁻ Tregs that produce IL-17 express CCR6 ex vivo. (A) Percentages of CCR6⁺ cells in gated ex vivo naive Tresps (n = 4), memory Tresps (n = 4), DR⁻ Tregs (n = 6) and DR⁺ Tregs (n = 6). (B) CCR6⁻ and CCR6⁺ fractions of DR⁻ Tregs or memory Tresps were FACS-sorted and stimulated for 5 days with αCD3/αCD28 and IL-2 in the presence of IL-1β/IL-6. The percentages of IL-17⁺ cells at the end of the culture is shown (n = 4). (C) Cells were stimulated in the presence of IL-1β/IL-6 or TGFβ, and analyzed for IL-17 secretion by ELISA. At the end of the culture, CCR6⁺DR⁻ Tregs were stained for intracellular IL-17 versus FoxP3 (D) or IFNγ (E). Data are representative of 4 independent experiments. **P < .01; *P < .05.

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