Figure 3
Figure 3. Expression of CD40 by B cells is critical for the αGalCer-induced enhancement of T-D B-cell response to protein vaccines. Specific IgG responses induced in mixed BMCs and wt mice upon vaccination with Ags mixed or not mixed with αGalCer, according to the schedule shown in Figure S1. (A) Comparative expression of CD40 on circulating B lymphocytes (CD19+) of BMCs and, in 2 animals displaying the expected expression on circulating B cells, on splenic DCs (CD11c+) of BMCs. (B) NP-specific antibody titers induced by vaccination of mixed BMCs and CD40−/− mice. Asterisks indicate statistically significant differences (P ≤ .05) in antibody titers between groups of mice immunized with Ag alone or mixed with αGalCer. Shown are the results from 1 experiment representative of 3 in which 5 mice per group were tested. Results are shown as mean plus or minus SEM.

Expression of CD40 by B cells is critical for the αGalCer-induced enhancement of T-D B-cell response to protein vaccines. Specific IgG responses induced in mixed BMCs and wt mice upon vaccination with Ags mixed or not mixed with αGalCer, according to the schedule shown in Figure S1. (A) Comparative expression of CD40 on circulating B lymphocytes (CD19+) of BMCs and, in 2 animals displaying the expected expression on circulating B cells, on splenic DCs (CD11c+) of BMCs. (B) NP-specific antibody titers induced by vaccination of mixed BMCs and CD40−/− mice. Asterisks indicate statistically significant differences (P ≤ .05) in antibody titers between groups of mice immunized with Ag alone or mixed with αGalCer. Shown are the results from 1 experiment representative of 3 in which 5 mice per group were tested. Results are shown as mean plus or minus SEM.

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