American Society of Hematology

Figure 2

$Figure 2. CD4+CD45RO+ immune memory to Nm antigens is absent in subjects with XLA. PBMC from XLA and healthy controls were depleted of CD45RA+ naive T cells. Undepleted (0.6 × 106/mL) and CD45RA+-depleted (0.3 × 106/mL) fractions were stimulated with Nm OMVs (H44/76, TR4, TR10, PorA−, LpxA−; 1 μg/mL) and influenza antigens (90 ng/mL). Proliferation was assessed by tritiated-thymidine (3HTdR) incorporation during days 3 to 9 of culture. Results are expressed as the mean number of triplicate corrected counts per minute (ccpm). The standard error of the mean cell counts was always less than 10% of the mean value shown. Data are representative of control n = 3 and includes all XLA patients n = 4.$

CD4⁺CD45RO⁺ immune memory to Nm antigens is absent in subjects with XLA. PBMC from XLA and healthy controls were depleted of CD45RA⁺ naive T cells. Undepleted (0.6 × 10⁶/mL) and CD45RA⁺-depleted (0.3 × 10⁶/mL) fractions were stimulated with Nm OMVs (H44/76, TR4, TR10, PorA⁻, LpxA⁻; 1 μg/mL) and influenza antigens (90 ng/mL). Proliferation was assessed by tritiated-thymidine (³HTdR) incorporation during days 3 to 9 of culture. Results are expressed as the mean number of triplicate corrected counts per minute (ccpm). The standard error of the mean cell counts was always less than 10% of the mean value shown. Data are representative of control n = 3 and includes all XLA patients n = 4.

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