Systemically administered BLP ameliorates cutaneous inflammation. (A) Hematoxylin and eosin (H&E) staining of untreated murine dorsal skin. E, epidermis; D, dermis; F, fatty layer; M, muscle layer. (B) WT mice were given daily intraperitoneal prophylactic doses of either (i) saline or (ii) 100 μg BLP, followed by 3 days of TPA painting to shaved dorsal skin (150 μL of a 50-μmol/L solution in acetone). Two days later mice were culled and skin sections stained by H&E. (C) D6-deficient mice were given daily intraperitoneal prophylactic doses of either (i) saline or (ii) BLP and subjected to TPA painting, as for WT mice. Four days after the TPA painting, mice were culled and skin sections stained by H&E. (D) D6-deficient mice were painted with TPA daily for 3 days and given (i) therapeutic saline or (ii) 100 μg BLP 48 hours after final TPA paint. All results are representative of 3 separate experiments with 5 mice per group per experiment.