Dasatinib inhibits MM tumor growth and angiogenesis in vivo. (A) Animals were killed when their tumors reached 2 cm in diameter or when paralysis or major compromise in their life occurred. Tumor volume is indicated as mean (± SD). (B) Survival was evaluated from the first day of treatment until death. (C) Representative microscopic images of tumor sections costained with hematoxylin and oesin and anti-CD31. (D) Angiogenic responses induced by gelatin sponges soaked with MM patient plasma cells (ii) or EC (iv) conditioned-medium compared with the sponge treated with medium alone (i). After treatment of the CAM with 50 nM dasatinib (iii and v, respectively), significantly fewer vessels surrounded the sponges (original magnifications ×50). Vessel counts are indicated.