Altered IL-17 and IFN-γ production in CD4⁺ T cells. Mesenteric LN cells from 3- to 4-week-old mice were stimulated with 50 ng/mL PMA and 500 ng/mL ionomycin for 5 hours, and cytokine-producing CD4⁺ T cells were measured by intracellular staining. (A) FACS profiles are representative data from one experiment. (B) Bar graphs represent mean plus or minus SD of 5 to 8 mice from 3 or 4 independent experiments. *P < .001; **P < .005; ***P < .05.